Protein Engineering

Engineering Novel Complement Activity into a Pulmonary Surfactant Protein

Complement activation / Biological Chemistry / Protein Engineering / Biological Sciences / Animals / Biological / Surface plasmon resonance / CHEMICAL SCIENCES / Rats / Substrate Specificity / Protein Conformation / Amino Acid Sequence / Structure activity Relationship / Recombinant Proteins / Pulmonary Surfactant / Molecular Sequence Data / Biological / Surface plasmon resonance / CHEMICAL SCIENCES / Rats / Substrate Specificity / Protein Conformation / Amino Acid Sequence / Structure activity Relationship / Recombinant Proteins / Pulmonary Surfactant / Molecular Sequence Data

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Antifungal Tradecraft by Cholesterol Oxidase

Organic Chemistry / Catalysis / Statistical Analysis / Protein Engineering / High throughput screening / Cholesterol / Pseudomonas / Enzyme / Substrate Specificity / Antifungal Agents / Chemical Properties / Chemistry Biology / Ergosterol / Function Space / Enzymatic Activity / Biochemistry and cell biology / Cholesterol / Pseudomonas / Enzyme / Substrate Specificity / Antifungal Agents / Chemical Properties / Chemistry Biology / Ergosterol / Function Space / Enzymatic Activity / Biochemistry and cell biology

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Protein Evolution via Amino Acid and Codon Elimination

Engineering / Physics / Chemistry / Synthetic Biology / Biology / Medicine / Gene expression / Multidisciplinary / Protein Engineering / Structural Stability / Mutagenesis / Protein evolution / Proteins / Green Fluorescent Protein / PLoS one / Steady state / Phenylalanine / Amino Acid Profile / Codon / Amino Acid Sequence / Genetic Code / Medicine / Gene expression / Multidisciplinary / Protein Engineering / Structural Stability / Mutagenesis / Protein evolution / Proteins / Green Fluorescent Protein / PLoS one / Steady state / Phenylalanine / Amino Acid Profile / Codon / Amino Acid Sequence / Genetic Code

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Enterotoxigenic Escherichia coli (K88) induce proinflammatory responses in porcine intestinal epithelial cells

Immunology / Inflammation / Protein Engineering / Diarrhea / Toll like receptor signaling / Cell line / Intestinal Mucosa / Escherichia coli / Animals / Developmental / Immune system / Swine / Virulence factor / Virulence Factors / Bacterial Adhesion / Escherichia Coli Infections / Interleukin / Flagellin / Cell line / Intestinal Mucosa / Escherichia coli / Animals / Developmental / Immune system / Swine / Virulence factor / Virulence Factors / Bacterial Adhesion / Escherichia Coli Infections / Interleukin / Flagellin

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Combinatorial Engineering of Dextransucrase Specificity

Catalysis / Magnetic Resonance Spectroscopy / Multidisciplinary / Protein Engineering / Mutation / Glucans / PLoS one / Substrate Specificity / Glycosyltransferases / Amino Acid Sequence / Structure activity Relationship / Site-directed Mutagenesis / Leuconostoc / Glucans / PLoS one / Substrate Specificity / Glycosyltransferases / Amino Acid Sequence / Structure activity Relationship / Site-directed Mutagenesis / Leuconostoc

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Design and characterization of a novel cellular prion-derived quenched fluorimetric substrate of α-secretase

Membrane Proteins / Protein Engineering / Cell line / Humans / Kidney / Mice / Animals / Biochemical / Prion disease / Substrate Specificity / Amino Acid Profile / Transfection / Human Fibroblasts / Serine Protease / Fluorometry / Biochemistry and cell biology / Prion Protein / Mice / Animals / Biochemical / Prion disease / Substrate Specificity / Amino Acid Profile / Transfection / Human Fibroblasts / Serine Protease / Fluorometry / Biochemistry and cell biology / Prion Protein

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A polyketide synthase acyltransferase domain structure suggests a recognition mechanism for its hydroxymalonyl-acyl carrier protein substrate

Engineering / Physics / Chemistry / Biology / Medicine / Multidisciplinary / Protein Engineering / Macromolecular X-Ray Crystallography / Peptides / PLoS one / Molecular cloning / Substrate Specificity / Multienzyme complexes / Bacillus Cereus / Multidisciplinary / Protein Engineering / Macromolecular X-Ray Crystallography / Peptides / PLoS one / Molecular cloning / Substrate Specificity / Multienzyme complexes / Bacillus Cereus

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N-terminal amphipathic helix as a trigger of hemolytic activity in antimicrobial peptides: A case study in latarcins

Biochemistry / Microbiology / Immunology / Molecular Biology / Computational Biology / Protein Engineering / Molecular modeling / Mutation / EC / Case Study / Animals / Antimicrobial Peptide / AMPs / Antimicrobial Cationic Peptides / Protein Secondary Structure Prediction / Cytotoxic Activity / Ec / Amino Acid Sequence / In Silico / Biochemistry and cell biology / Hemolysis / Proof of Concept / Protein Engineering / Molecular modeling / Mutation / EC / Case Study / Animals / Antimicrobial Peptide / AMPs / Antimicrobial Cationic Peptides / Protein Secondary Structure Prediction / Cytotoxic Activity / Ec / Amino Acid Sequence / In Silico / Biochemistry and cell biology / Hemolysis / Proof of Concept

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Design and bioproduction of a recombinant multi(bio)functional elastin-like protein polymer containing cell adhesion sequences for tissue engineering purposes

Materials Engineering / Biomedical Engineering / Tissue Engineering / Cell Adhesion / Materials / Polymers / Extracellular Matrix / Protein Engineering / Genetic Engineering / Escherichia coli / Peptides / Elastin / Biomimetic materials / Amino Acid Profile / Protein Conformation / Molecular weight / Cell Proliferation / Amino Acid Sequence / Materials Testing / Recombinant Proteins / Biocompatible Materials / Cell Adhesion Molecules / Polymers / Extracellular Matrix / Protein Engineering / Genetic Engineering / Escherichia coli / Peptides / Elastin / Biomimetic materials / Amino Acid Profile / Protein Conformation / Molecular weight / Cell Proliferation / Amino Acid Sequence / Materials Testing / Recombinant Proteins / Biocompatible Materials / Cell Adhesion Molecules

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Designer laccases: a vogue for high-potential fungal enzymes?

Engineering / Technology / Gene expression / Protein Engineering / Genetic Engineering / Biological Sciences / Industrial microbiology / TRENDS / Laccase / Enzyme / Industrial Production / Recombinant Proteins / Ascomycota / Site-directed Mutagenesis / BASIDIOMYCOTA / Biological Sciences / Industrial microbiology / TRENDS / Laccase / Enzyme / Industrial Production / Recombinant Proteins / Ascomycota / Site-directed Mutagenesis / BASIDIOMYCOTA

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Design and bioproduction of a recombinant multi(bio)functional elastin-like protein polymer containing cell adhesion sequences for tissue engineering purposes

Materials Engineering / Biomedical Engineering / Tissue Engineering / Cell Adhesion / Materials / Polymers / Extracellular Matrix / Protein Engineering / Genetic Engineering / Escherichia coli / Peptides / Elastin / Biomimetic materials / Amino Acid Profile / Protein Conformation / Molecular weight / Cell Proliferation / Amino Acid Sequence / Materials Testing / Recombinant Proteins / Biocompatible Materials / Cell Adhesion Molecules / Polymers / Extracellular Matrix / Protein Engineering / Genetic Engineering / Escherichia coli / Peptides / Elastin / Biomimetic materials / Amino Acid Profile / Protein Conformation / Molecular weight / Cell Proliferation / Amino Acid Sequence / Materials Testing / Recombinant Proteins / Biocompatible Materials / Cell Adhesion Molecules

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High-efficiency protein expression mediated by enterovirus 71 internal ribosome entry site

Biotechnology / Multidisciplinary / Protein Engineering / Humans / Genetic Enhancement / Animals / Hepatocellular Carcinoma / Protein Expression / Transfection / Ribosomes / High Efficiency / Internal ribosome entry site / Protein Biosynthesis / Animals / Hepatocellular Carcinoma / Protein Expression / Transfection / Ribosomes / High Efficiency / Internal ribosome entry site / Protein Biosynthesis

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Engineering a terbium-binding site into an integral membrane protein for luminescence energy transfer

Fluorescence / Multidisciplinary / Protein Engineering / Fluorescence Resonance Energy Transfer / Mutation / Escherichia coli / Terbium / Membrane transport proteins / Protein Secondary Structure Prediction / Spectrum analysis / Energy Transfer / Membrane Protein / Structure activity Relationship / Cytoplasm / Binding Site / Escherichia coli / Terbium / Membrane transport proteins / Protein Secondary Structure Prediction / Spectrum analysis / Energy Transfer / Membrane Protein / Structure activity Relationship / Cytoplasm / Binding Site

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Enterotoxigenic Escherichia coli (K88) induce proinflammatory responses in porcine intestinal epithelial cells

Immunology / Inflammation / Protein Engineering / Diarrhea / Toll like receptor signaling / Cell line / Intestinal Mucosa / Escherichia coli / Animals / Developmental / Immune system / Swine / Virulence factor / Virulence Factors / Bacterial Adhesion / Escherichia Coli Infections / Interleukin / Flagellin / Cell line / Intestinal Mucosa / Escherichia coli / Animals / Developmental / Immune system / Swine / Virulence factor / Virulence Factors / Bacterial Adhesion / Escherichia Coli Infections / Interleukin / Flagellin

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Crucial Optimization of Translational Components towards Efficient Incorporation of Unnatural Amino Acids into Proteins in Mammalian Cells

Multidisciplinary / Protein Engineering / Mammals / Humans / Animals / Proteins / Plasmids / PLoS one / Amino Acids / Substrate Specificity / Amino Acid Sequence / Base Sequence / Nucleic Acid Conformation / Protein Biosynthesis / Proteins / Plasmids / PLoS one / Amino Acids / Substrate Specificity / Amino Acid Sequence / Base Sequence / Nucleic Acid Conformation / Protein Biosynthesis

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A biotechnological method provides access to aggregation competent monomeric Alzheimer\'s 1-42 residue amyloid peptide

Physical Chemistry / Electron Microscopy / Mass Spectrometry / Gene expression / Multidisciplinary / Protein Engineering / Humans / Mutagenesis / Escherichia coli / Bio Technology / Animals / High Pressure Liquid Chromatography / Solutions / Plasmodium falciparum / Amino Acid Sequence / Protein Engineering / Humans / Mutagenesis / Escherichia coli / Bio Technology / Animals / High Pressure Liquid Chromatography / Solutions / Plasmodium falciparum / Amino Acid Sequence

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